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Genet. mol. biol ; 29(2): 287-289, 2006.
Article in English | LILACS | ID: lil-432700

ABSTRACT

The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect alfa-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon I of the a-lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These a-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism (SNP) markers for association studies and marker assisted selection.


Subject(s)
Animals , Goats/genetics , Lactalbumin , Polymorphism, Single-Stranded Conformational , Exons/genetics , Genetic Variation , Genetics, Population , India , Polymerase Chain Reaction
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